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sureselect strand-specific rna library prep illumina multiplexed sequencing kit  (Agilent technologies)


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    Structured Review

    Agilent technologies sureselect strand-specific rna library prep illumina multiplexed sequencing kit
    Sureselect Strand Specific Rna Library Prep Illumina Multiplexed Sequencing Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sureselect+strand-specific+rna+library+prep+kit/10__1007_slash_s11105___024___01452___7-70-6-16
    Average 90 stars, based on 1 article reviews
    sureselect strand-specific rna library prep illumina multiplexed sequencing kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Resilience to aging in the regeneration-capable flatworm Macrostomum lignano.
    Article Snippet: Total RNA was extracted from the samples with the Direct-zol RNA MiniPrep Kit (Zymo Research), following the manufacturer’s protocol. .. The concentration of the extracted RNA was assessed using the Qubit RNA BR Assay Kit (Life Technologies), and RNA-Seq libraries were generated with the SureSelect Strand-Specific RNA Library Prep Kit for Illumina multiplexed sequencing (Agilent) in accordance with the manufacturer’s instructions. .. Libraries were pooled, 16 samples per run (2 nM), and sequenced on the Illumina HiSeq 2500 machine.

    RNA sequencing:

    Article Title: Resilience to aging in the regeneration-capable flatworm Macrostomum lignano.
    Article Snippet: Total RNA was extracted from the samples with the Direct-zol RNA MiniPrep Kit (Zymo Research), following the manufacturer’s protocol. .. The concentration of the extracted RNA was assessed using the Qubit RNA BR Assay Kit (Life Technologies), and RNA-Seq libraries were generated with the SureSelect Strand-Specific RNA Library Prep Kit for Illumina multiplexed sequencing (Agilent) in accordance with the manufacturer’s instructions. .. Libraries were pooled, 16 samples per run (2 nM), and sequenced on the Illumina HiSeq 2500 machine.

    Article Title: Multifaceted analysis of cross-tissue transcriptomes reveals phenotype-endotype associations in atopic dermatitis.
    Article Snippet: Library preparation was carried out using NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions. .. For PBMC RNAseq, RNAwas isolated usingMaxwell 16 LEV simplyRNA Blood Kit and Maxwell 16 Instrument (Promega) and library preparation were carried out with SureSelect Strand-Specific RNA Library Prep Kit (Agilent). .. The libraries were pooled for skin tissue RNA-seq and PBMC RNA-seq, respectively, and sequenced on HiSeq1500 or HiSeq2500 with bcl2fastq (Illumina) to obtain 15–20 million reads using the 50- bp single-end read configuration.

    Generated:

    Article Title: Resilience to aging in the regeneration-capable flatworm Macrostomum lignano.
    Article Snippet: Total RNA was extracted from the samples with the Direct-zol RNA MiniPrep Kit (Zymo Research), following the manufacturer’s protocol. .. The concentration of the extracted RNA was assessed using the Qubit RNA BR Assay Kit (Life Technologies), and RNA-Seq libraries were generated with the SureSelect Strand-Specific RNA Library Prep Kit for Illumina multiplexed sequencing (Agilent) in accordance with the manufacturer’s instructions. .. Libraries were pooled, 16 samples per run (2 nM), and sequenced on the Illumina HiSeq 2500 machine.

    Sequencing:

    Article Title: Resilience to aging in the regeneration-capable flatworm Macrostomum lignano.
    Article Snippet: Total RNA was extracted from the samples with the Direct-zol RNA MiniPrep Kit (Zymo Research), following the manufacturer’s protocol. .. The concentration of the extracted RNA was assessed using the Qubit RNA BR Assay Kit (Life Technologies), and RNA-Seq libraries were generated with the SureSelect Strand-Specific RNA Library Prep Kit for Illumina multiplexed sequencing (Agilent) in accordance with the manufacturer’s instructions. .. Libraries were pooled, 16 samples per run (2 nM), and sequenced on the Illumina HiSeq 2500 machine.

    Article Title: Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity
    Article Snippet: The concentration of purified RNA was measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and its quality was assessed with a 2100 Bioanalyzer (Agilent). .. The total RNA (1μg) was converted into cDNA library for Illumina multiplex sequencing by using SureSelect Strand-Specific RNA Library Prep kit (Agilent) in accordance with the manufacturer’s protocol. .. The fragment length and quality of cDNA library was assessed with a 2100 Bioanalyzer, and the concentration was determined by using KAPA library quantification Kit (NIPPON Genetics).

    other:

    Article Title: Repeated inversions within a pannier intron drive diversification of intraspecific colour patterns of ladybird beetles
    Article Snippet: RNA-seq library preparation was performed using the SureSelect strand-specific RNA library prep kit (Agilent) according to the manufacturer’s instructions.

    Article Title: Identification of Genes Involved in the Synthesis of the Fungal Cell Wall Component Nigeran and Regulation of Its Polymerization in Aspergillus <i>luchuensis</i>
    Article Snippet: Total RNA (150 ng) was used for cDNA synthesis using a SureSelect strand-specific RNA library prep kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Expression of mitochondrial protein genes encoded by nuclear and mitochondrial genomes correlate with energy metabolism in dairy cattle
    Article Snippet: RNA Integrity Numbers (RIN), which indicates the RNA quality, were determined using Agilent Tapestation (Agilent) and RNAseq libraries were prepared from all samples (Additional le 1) with RIN > 6 at which the 3’ bias level is at a minimum using the SureSelect Strand-Speci c RNA Library Prep Kit (Agilent) as instructed by the manufacturer.

    Article Title: Distribution and Phylogeny of Erythrocytic Necrosis Virus (ENV) in Salmon Suggests Marine Origin
    Article Snippet: Approximately 200 ng of total RNA was lyophilized and fed into the SureSelect Strand-Specific RNA library Prep kit (Agilent, Santa Clara, CA, USA) according to the manufacturer’s instructions.

    cDNA Library Assay:

    Article Title: Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity
    Article Snippet: The concentration of purified RNA was measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and its quality was assessed with a 2100 Bioanalyzer (Agilent). .. The total RNA (1μg) was converted into cDNA library for Illumina multiplex sequencing by using SureSelect Strand-Specific RNA Library Prep kit (Agilent) in accordance with the manufacturer’s protocol. .. The fragment length and quality of cDNA library was assessed with a 2100 Bioanalyzer, and the concentration was determined by using KAPA library quantification Kit (NIPPON Genetics).

    Multiplex Assay:

    Article Title: Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity
    Article Snippet: The concentration of purified RNA was measured with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and its quality was assessed with a 2100 Bioanalyzer (Agilent). .. The total RNA (1μg) was converted into cDNA library for Illumina multiplex sequencing by using SureSelect Strand-Specific RNA Library Prep kit (Agilent) in accordance with the manufacturer’s protocol. .. The fragment length and quality of cDNA library was assessed with a 2100 Bioanalyzer, and the concentration was determined by using KAPA library quantification Kit (NIPPON Genetics).

    Isolation:

    Article Title: Multifaceted analysis of cross-tissue transcriptomes reveals phenotype-endotype associations in atopic dermatitis.
    Article Snippet: Library preparation was carried out using NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions. .. For PBMC RNAseq, RNAwas isolated usingMaxwell 16 LEV simplyRNA Blood Kit and Maxwell 16 Instrument (Promega) and library preparation were carried out with SureSelect Strand-Specific RNA Library Prep Kit (Agilent). .. The libraries were pooled for skin tissue RNA-seq and PBMC RNA-seq, respectively, and sequenced on HiSeq1500 or HiSeq2500 with bcl2fastq (Illumina) to obtain 15–20 million reads using the 50- bp single-end read configuration.



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